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KMID : 0364820100460040389
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Korean Journal of Microbiology
2010 Volume.46 No. 4 p.389 ~ p.396
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Production of Microbial Insecticide Using Bacillus thuringiensis BT17 for the Control of Lepidopteran Larvae
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Ahn Kyung-Joon
Lee Tae-Geun
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Abstract
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Insecticidal crystalline toxin producing Bacillus thuringiensis BT17 strain was isolated and identified as B. thuringiensis serovar colmeri by 16S rRNA analysis. BT17 strain produced crystalline δ-endotoxin against to Lepidopteran larvae effectively on the culture broth of soybean meal and skim milk, 30oC and 36 h shaking culture of 280 rpm. The maximum colony forming unit achieved when the culture was continued for 24 h, but the number of crystals increased until 36 h in the 200 L fermentor. Liquid type of biological insecticide product was made, and after 3 months storage in 20oC the
number of crystals was increased up to twice than beginning. Biocontrol effect of BT17 insecticide product was better in Plutella xylostella than in Spodoptera exigua, and the toxicity to animals was negligible.
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KEYWORD
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B. thuringiensis, δ-endotoxin, biocontrol, Lepidoptera, microbial insecticide
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